教員プロフィール

藤井 儀夫

藤井 儀夫

Fujii Yoshio

臨床薬学科/機能形態学研究室(教授)

最終学歴

岡山大学大学院 薬学研究科 修士課程 修了

取得学位

薬学博士 (徳島文理大学)

所属学会

日本薬学会、日本細菌学会、日本薬理学会、アメリカ細菌学会

現在の専門分野

細菌学、細菌毒素、細胞生物学

受賞

日本細菌学会 黒屋奨学賞

現在の研究課題

アエロモナス菌の産生する溶血毒素の研究
アエロモナス菌の産生するプロテアーゼの研究
毒素原性大腸菌の産生する耐熱性下痢毒素II(STII)の研究

主要研究業績

論文 Y. Fujii, K. Tsurumi, M. Sato, E. Takahashi, and K. Okamoto.
Fluid secretion caused by aerolysin-like hemolysin of
Aeromonas sobria in the intestines is due to stimulation of
production of prostaglandin E2 via cyclooxygenase-2 by
the intestinal cells.
Infect. Immun. 76, 1076-1082 (2008).

 

論文 Y. Fujii, T. Nomura, R. Yokoyama, S. Shinoda, and K. Okamoto.
Studies of the mechanism of action of the aerolysin-like hemolysin of
Aeromonas sobria in stimulating T84 cells to produce cyclic AMP.
Infect. Immun. 71, 1557-1560 (2003).

 

論文 Y. Fujii, T. Nimura, and K. Okamoto.
Hemolysin of Aeromonas sobria stimulates production of cyclic AMP
by cultured cells.
FEMS Microbiol. Lett. 176, 67-72 (1999).

 

論文 Y. Fujii, T. Nomura, H. Kanzawa, M. Kameyama, H. Yamanaka, M. Akita,
K. Setsu, and K. Okamoto.
Purification and characterization of enterotoxin produced by Aeromonas sobria.
Microbiol. Immunol. 42, 703-714 (1998).

 

論文 Y. Fujii, Y. Kondo, and K. Okamoto.
Involvement of prostaglandin E2 synthesis in the intestinal secretory action of
Escherichia coli heat-stable enterotoxin II.
FEMS Microbiol. Lett.. 130, 259-266 (1995).

 

論文 Y. Fujii, Y. Okamuro, S. Hitotsubashi, A. Saito, N. Akashi, and K. Okamoto.
Effect of alterations of basic amino acid residues of Escherichia coli
heat-stable enterotoxin II on enterotoxicity.
Infect. Immun. 62, 2295-2301 (1994).

 

論文 Y. Fujii, M. Hayashi, S. Hitostubashi, Y. Fuke, H. Yamanaka, and K. Okamoto.
Purification of characterization of Escherichia coli heat-stable enterotoxin II.
J. Bacteriol. 173, 5516-5522 (1991).

最近の研究業績
  1. E. Takahashi, H. Ozaki, Y. Fujii, H. Kobayashi, H. Yamanaka, S. Arimoto, T. Negishi, K. Okamoto.
    Properties of hemolysin and protease produced by Aeromonas trota.
    PLoS ONE. 9(3): e91149. doi: 10.1371/journal.pone.0091149 (2014).

  2. E. Takahashi, Y. Fujii, H. Kobayashi, H. Yamanaka, G. B. Nair,
    Y. Takeda, S. Arimoto, T. Negishi, and K. Okamoto.
    Maturation pathway of metalloprotease produced
    by Aeromonas sobria.
    Microbiol. Immunol. 54, 596-605 (2010).

  3. Y. Fujii, K. Tsurumi, M. Sato, E. Takahashi, and K. Okamoto.
    Fluid secretion caused by aerolysin-like hemolysin of
    Aeromonas sobria in the intestines is due to stimulation of
    production of prostaglandin E2 via cyclooxygenase-2 by
    the intestinal cells.
    Infect. Immun. 76, 1076-1082 (2008).

  4. 藤井儀夫 (共著)
    べーシック薬学教科書シリーズ
    機能形態学, 化学同人 (2008).

  5. H. Kobayashi, E. Takahashi, K. Oguma, Y. Fujii, H. Yamanaka,
    T. Negishi, S. Arimoto-Kobayashi, T. Tsuji, and K. Okamoto.
    Cleavage specificity of the serine protease of Aeromonas sobria,
    a member of the kexin family of subtilases.
    FEMS Micorobiol. Lett. 256, 165-170 (2006).

  6. A. Takahashi, M. Nakano, K. Okamoto, Y. Fujii, K. Mawatari,
    N. Harada, and Y. Nakaya.
    Aeromonas sobria hemolysin causes diarrhea by increasing
    secretion of HCO3-.
    FEMS Micorobiol. Lett. 258, 92-95 (2006).

  7. A. Takahashi, N. Tanoue, M. Nakano, A. Hamamoto, K. Okamoto,
    Y. Fujii, N. Harada, and Y. Nakaya.
    A pore-forming toxin produced by Aeromonas sobria activates
    Ca (2+) dependent Cl (-) secretion.
    Microb. Pathog. 38, 173-180 (2005).

  8. N. Tanoue, A. Takahashi, K. Okamoto, Y. Fujii, Y. Taketani,
    N. Harada, M. Nakano, and Y. Nakaya.
    A pore-forming toxin produced by Aeromonas sobria activates
    cAMP-dependent Cl-secretory pathways to cause diarrhea.
    FEMS Microbiol. Lett. 242, 195-201 (2005).

  9. Y. Li, K. Okamoto, E. Takahashi, S. Miyoshi, S. Shinoda, T. Tsuji,
    and Y. Fujii.
    A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce
    ATP and cyclic AMP which appear to be secretory mediators.
    Microbiol. Immunol. 49, 73-78 (2005).

  10. A. Wada, Ai-P. Wanga, H. Isomoto, Y. Satomi, T. Takao, A. Takahashi,
    S. Awata, T. Nomura, Y. Fujii, S. Kohno, K. Okamoto, J. Moss,
    J. L. Millan, and T. Hirayama.
    Placental and intestinal alkaline phosphatases are receptors for
    Aeromonas sobria hemolysin.
    Int. J. Med. Microbiol. 294, 427-435 (2005).

  11. Y. Fujii, T. Nomura, R. Yokoyama, S. Shinoda, and K. Okamoto.
    Studies of the mechanism of action of the aerolysin-like hemolysin
    of Aeromonas sobria in stimulating T84 cells to produce cyclic AMP.
    Infect. Immun. 71, 1557-1560 (2003).

  12. T. Funahashi, T. Tanabe, H. Aso, H. Nakao, Y. Fujii, K. Okamoto,
    S. Narimatsu, and S. Yamamoto.
    An iron-regulated gene required for utilization of aerobactin as
    an exogenous siderophore in Vibrio parahaemolyticus.
    Microbiology. 149, 1217-1225 (2003).

  13. Y. Fujii, S. Sugiura, S. Yamamoto, and K. Okamoto.
    Purification and some properties of hemolysin produced by
    Aeromonas sobria. In K. Setsu and C-H. Roan (ed.).
    Advancement of Life Science. AMVO publishing company.
    Taiwan, pp 87-92. (2003).

  14. Y. Fujii, K. Tsurumi, R. Yokoyama, and K. Okamoto.
    Aeromonas sobria hemolysin stimulates production of
    cyclic AMP in T84 cells. In K. Setsu and C-H. Roan (ed.).
    Advancement of Life Science. AMVO publishing company.
    Taiwan, pp 93-98. (2003).

  15. R. Yokoyama, Y. Fujii, Y. Noguchi, T. Nomura, M. Akita, K. Setsu,
    S. Yamamoto, and K. Okamoto.
    Physicochemical and biological propertied of an extracellular
    serine protease of Aeromonas sobria.
    Microbiol. Immunol. 46, 383-390 (2002).

  16. M. Watarai, S. Makino, Y. Fujii, K. Okamoto, and T. Shirahata.
    Modulation of Brucella-induced macropinocytosis by lipid rafts
    mediates intracellular replication.
    Cellular Microbiol. 4, 341-355 (2002).

  17. T. Nomura, Y. Fujii, H. Yamanaka, H. Kobayashi, and K. Okamoto.
    The protein incoded at the 3' end of the serine protease gene of
    Aeromonas sobria functions as a chaperone in the production of
    the protease.
    J. Bacteriol. 184, 7058-7061 (2002).

  18. 岡本敬の介, 藤井儀夫
    細菌毒素ハンドブック
     アエロモナス ソブリア溶血毒素,
     サイエンスフォーラムpp 316-321. (2002).

  19. K. Okamoto, H. Yamanaka, M. Takeji, and Y. Fujii.
    Region of heat-stable enterotoxin II of Escherichia coli involved
    in translocation across the outer membrane.
    Microbiol. Immunol. 45, 349-355 (2001).

  20. 岡本敬の介, 藤井儀夫
     化学と生物 実験ライン48
     タンパク質と核酸の分離精製, 廣川書店 (2001).

  21. K. Okamoto, T. Nomura, M. Hamada, T. Fukuda, Y. Noguchi,
    and Y. Fujii.
    Production of serine protease of Aeromonas sobria is controlled
    by the protein encoded by the gene lying adjacent to the 3'end of
    the protease gene.
    Microbiol. Immunol. 44, 787-798 (2000).

  22. Y. Fujii, T. Nomura, and K. Okamoto.
    Hemolysin of Aeromonas sobria stimulates production of
    cyclic AMP by cultured cells.
    FEMS Microbiol. Lett. 176, 67-72 (1999).

  23. T. Nomura, Y. Fujii, and K. Okamoto.
    Secretion of hemolysin of Aeromonas sobria as protoxin and
    contribution of the propeptide region removed from the protoxin
    to the proteolytic stability of the toxin.
    Microbiol. Immunol. 43, 29-38 (1999).

  24. A.-P. Wang, A. Wada, K. Yahiro, T. Nomura, Y. Fujii, K. Okamoto,
    Y. Mizuta, S. Kohno, J. Moss, and T. Hirayama.
    Identification and characterization of the Aeromonas sobria
    hemolysin glycoprotein receptor on intestine 407 cells.
    Microb. Pathog. 27, 215-221 (1999).

  25. Y. Fujii, T. Nomura, H. Kanzawa, M. Kameyama, H. Yamanaka,
    M. Akita, K. Setsu, and K. Okamoto.
    Purification and characterization of enterotoxin produced by
    Aeromonas sobria.
    Microbiol. Immunol. 42, 703-714 (1998).

  26. K. Okamoto, T. Nomura, Y. Fujii, and H. Yamanaka.
    Contribution of the disulfide bond of the A subunit to the action
    of Escherichia coli heat-stable enterotoxin.
    J. Bacteriol. 180, 1368-1374 (1998).

  27. H. Yamanaka, T. Nomura, Y. Fujii, and K. Okamoto.
    Need for Tol C, Escherichia coli outer membrane protein, in the
    secretion of heat-stable enterotoxin I across the outer membrane.
    Microb. Pathog. 25, 111-120 (1998).