教員プロフィール

中条　茂男

中条　茂男
Nakajo Shigeo

健康薬学科／生化学研究室 (教授)
 

◆最終学歴
昭和大学大学院薬学研究科（前期課程）

 

◆取得学位
博士（薬学）

 

◆所属学会
日本薬学会 、日本生化学会 、日本神経化学会 、日本癌学会、Society For Neuroscience(米国)

 

◆現在の専門分野
細胞分子生物学

 

◆現在の研究課題
・Neural stem cells（神経幹細胞）の自己増殖能、および分化機構に関する研究
・ヒト白血病細胞や癌細胞の細胞死の分子機構に関する研究

 

◆主要研究業績
・神経幹細胞の分化機構に関する研究
・神経特異的タンパク質PNP (Phosphoneuroprotein) 14（PNP 14, β-synuclein）の発見、
構造解析、および機能に関する研究
・ヒト白血病細胞、癌細胞の分化・細胞死に関する研究
・味変換タンパク質（ミラクリン、クルクリン）の構造と性質に関する研究
・cAMP-非依存性プロテインキナーゼの構造と機能に関する研究

 

◆最近の研究業績

  1. Pituitary adenylate cyclase-activating polypeptide-induced differentiation of embryonic neural stem cells into astrocytes is mediated via the β isoform of protein kinase C.
    Watanabe, J., Ohba, M., Ohno, F., Kikuyama, S., Nakamura, M., Nakaya, K., Arimura, A., Shioda, S., and Nakajo, S. (2006) J. Neurosci. Res., 84, 1645-1655.
  2. Differentiation of neural stem cells into astrocytes by low concentration of pituitary adenylate cyclase-activating polypeptide (PACAP). In: Homma I and Shioda S (eds) Breathing, Feeding, and Neuroprotection.
    Watanabe, J., Shioda, S., Kikuyama, S., Nakaya, K., and Nakajo, S. (2006) Springer-Verlag, Tokyo, pp 57-63.
  3. Involvement of Tiam1 in apoptosis induced by bufalin in HeLa cells.
    Hong, C., Shibayama-Imazu, T., Masuda, Y., Shinki, T., Nakajo, S., and Nakaya, K. (2006) Anticancer Res., 26, 245-250.
  4. Involvement of protein kinase C in the PACAP-induced differentiation of neural stem cells into astrocytes.
    Watanabe, J., Ohno, F., Shioda, S., Kikuyama, S., Nakaya, K. and Nakajo, S. (2006) Ann. NY. Acad. Sci., 1070, 597-601.
  5. Production of superoxide and dissipation of mitochondrial transmembrane potential by vitamin K2 trigger apoptosis in human ovarian cancer TYK-nu cells.
    Shibayama-Imazu, T., Sonoda, I., Sakairi, S., Aiuchi, T., Ann, WW., Nakajo, S., Itabe, H., and  Nakaya, K. (2006) Apoptosis, 11, 1535-1543.
  6. Pleiotropic functions of PACAP in the CNS.
    Shioda, S., Ohtaki, H., Nakamachi, T., Dohi, K., Watanabe, J., Nakajo, S., Arata, S., Kitamura, S.,  
    Okuda, H., Takenoya, F., and Kitamura, Y. (2006) Ann. NY. Acad. Sci., 1070, 550-560.
  7. Involvement of β isoform of PKC in PACAP-induced differentiation of neural stem cells into astrocytes.
    Nakajo, S., Watanabe, J., Ohba, M., Ohno, F., Kikuyama, S., Nakaya, K. and Shioda, S., In Kusano, M., and Shioda, S., editors. New Frontiers in Regenerative Medicine, Springer-Verlag Tokyo, (2006) p 121-134.
  8. A novel 21-kDa cytochrome c-releasing factor is generated upon treatment of human leukemia U937 cells with geranylgeraniol.
    Masuda, Y., Maeda, S., Watanabe, A., Sano, Y., Aiuchi, T., Nakajo, S., Itabe, H., and Nakaya, K. (2006) Biochem. Biophys. Res. Commun., 346, 454-460.
  9. Expression of estrogen receptor alpha, retinoic acid receptor alpha and cellular retinoic acid binding protein II genes is coordinately regulated in human breast cancer cells.
    Lu, M., Mira-L-Lopez., Nakajo, S., Nakaya, K., and Jing, Y. (2005) Oncogene, 24, 4362-4369.
  10. Pituitary adenylate cyclase-activating polypeptide (PACAP) promotes differentiation of mouse neural stem cells to astrocytes.
    Ohno, F., Watanabe, J., Sekihara, H., Hirabayashi, T., Arata, S., Kikuyama, S., Shioda, S.,
    Nakaya, K, and Nakajo, S. (2005) Regul. Peptides, 126, 115-122..
  11. Cellular retinol-binding protein, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity.
    Farias, E., Ong, D., Ghyselinck, N., Kuppumbatti, Y., Nakajo, S. and Mira-Y-Lopez, R. (2004)
    J. Natl. Cancer I., 97, 21-29.
  12. Involvement of tumor necrosis factor receptor-associated protein 1 (TRAP1) in apoptosis induced by β-hydroxyisovalerylshikonin.
    Masuda, Y., Shima, G., Auichi, T., Horie, M., Hori, K., Nakajo, S., Kajimoto, S., Shibayama-
    Imazu, T. and Nakaya, K. (2004) J. Biol. Chem., 279, 42503-42515.
  13. Regulation of cytosolic prostaglandin E synthase by phosphorylation.
    Kobayashi ,T., Nakatani, Y., Tanioka, T., Tsujimoto, M., Nakajo, S., Nakaya, K., Murakami, M., and Kudo, I. (2004) Biochem. J., 381, 59-69.
  14. Reducing activity of colloidal platinum nanoparticles for hydrogen peroxide, 2,2-diphenyl-1-picrylhydrazyl radical and 2,6-dichlorophenol indophenol.
    Aiuchi, T, Nakajo, S, and Nakaya, K. (2004) Biol. Pharm. Bull., 27, 736-738.
  15. Epigenetic CRBP downregulation appears to be an evolutionarily conserved (human and mouse) and oncogene-specific phenomenon in breast cancer.
    Arapshian, A., Bertran, S., Kuppumbatti YS., Nakajo, S., and Mira-Y-Lopez, R. (2004) Mol.
    Cancer, 3, 13.
  16. PACAPによる神経細胞死防御とその分子制御機構
    塩田清二、大滝博和、鈴木隆介、中町智哉、竹ノ谷文子、土肥謙二、中条茂男（2004）
    日本薬理学雑誌　123、243-252
  17. β-Hydroxyisovalerylshikonin and cisplatin act synergistically to inhibit growth and to induce apoptosis of human lung cancer DMS114 cells via a tyrosine kinase-dependent pathway.
    Xu, Y., Kajimoto, S., Nakajo, S., and Nakaya, K. (2004) Oncology-Basel, 66,67-75.
  18. Expression of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC1-R) in reactive astrocytes.
    Suzuki, R., Arata, S., Nakajo, S., Ikenaka, K., Kikuyama, S., and Shioda, S. (2003) Mol. Brain Res., 115, 10-20.
  19. Vitamin K2 selectively induced apoptosis in ovarian TYK-nu and pancreatic MIA PaCa-2 cells out of eight solid tumor cell lines through a mechanism different from geranylgeraniol.
    Shibayama-Imazu, T., Sakairi, S., Watanabe, A., Aiuchi, T., Nakajo, S., and Nakaya, K. (2003) J. Cancer Res. Clin. Oncol., 129, 1-11.
  20. β-Hydroxyisovalerylshikonin induces apoptosis in human leukemia cells by inhibiting the activity of a polo-like kinase 1 (PLK1).
    Masuda, Y., Nishida, A., Hori, K., Hirabayashi, T., Kajimoto, S., Nakajo, S., Kondo, T., Asaka, M., and Nakaya, K. (2003) Oncogene, 22, 1012-1023.
  21. Pituitary adenylate cyclase-activating polypeptide (PACAP) prevents hippocampal neurons from apoptosis by inhibiting JNK/SAPK and p38 signal transduction pathways.
    Dohi, K., Mizushima, H., Nakajo, S., Ohtaki, H., Matsunaga, S., Aruga, T., and Shioda, S. (2002)
    Regul. Pept., 109, 83-88.
  22. Effect of β-hydroxyisovalerylshikon and cancer chemothrerapeutic agents on leukemia U937 cells and lung cancer DM114 cells in vitro.
    Xu, Y., Nakajo, S., and Nakaya, K. (2002) Showa Univ. Med. Sci., 14, 241-247.
  23. Synergistic inhibitory effects of transplatin and β-hydroxyisovalerylshikon on carcinoma A431 cells involve epidermal growth factor receptor.
    Xu, Y., Nakajo, S., and Nakaya, K. (2002) Cancer Lett., 188, 67-72.
  24. Sophoranone, extracted from a traditional Chinese medicine Shan Dou Gen, induces apoptosis in human leukemia U937 cells via formation of reactive oxygen species and opening of mitochondorial permeability transition pores.
    Kajimoto, S., Takanashi, N., Kajimoto, T., Xu, M., Cao, J., Masuda, Y., Aiuchi, T., Nakajo, S., Ida, Y., and Nakaya, K, (2002) Int. J. Cancer, 99, 879-890.
  25. Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat
    hypothalamus.
    Suzuki R., Shimojima, H., Funahashi, H., Nakajo, S., Yamada, S., Guan, JL., Tsurugano, S,
    Uehara, K., Takeyama, Y., Kikuyama, S., and Shioda, S.(2002) Neurosci. Lett., 324, 5-8.
  26. β-Hydroxyisovalerylshikonin is a novel and potent inhibitor of protein tyrosine kinases.
    Kajimoto, S., Xu, Y., Masuda, Y., Aiuchi, T., Nakajo, S., Uehara, Y., Shibuya, M., Yamori, T.,
    and Nakaya, K. (2002) Jpn. J. Cancer Res., 93, 944-951.
  27. Reduced postischemic apoptosis in the hippocampus of mice deficient in interleukin-1.
    Mizushima, H., Zhou, C. J., Dohi, K., Horai, R., Asano, M., Iwakura, Y., Hirabayashi, T., Arata, S.,
    Nakajo, S., Takaki, A., Ohtaki, H., and Shioda, S. (2002) J. Comp. Neurol., 448, 203-16.
  28. CRBP suppresses breast cancer cell survival and anchorage-independent growth.
    Kappumbatti, Y-S., Rexer, B., Nakajo, S., Nakaya, K., and Mira-y-Lopez, R. (2001) Oncogene, 20,7413-7419.
  29. Expression of β-synuclein in normal human astrocytes.
    Tanji, K., Mori, F., Nakajo, S., Imaizumi, T., Yoshida, H., Hirabayashi, T., Yoshimoto, M., Satoh, K., Takahashi, H., and Wakabayashi, K. (2001), Neuroreport, 12, 2845-2848.
  30. Apoptosis induced by arsenic trioxide in U937 cells is dependent on activation of p38, inactivation of ERK and the Ca2+-dependent production of superoxide.
    Iwama, K., Nakajo, S., Aiuchi, T., and Nakaya, K. (2001) Int. J. Cancer, 92, 518-526.
  31. Cosecretion of prolactin and growth hormone by dispersed pituitary cells of the adult bullfrog, Rana catesbeiana.
    Oguchi, A., Aida, T., Koda, A., Shioda, S., Nakajo, S., Kobayashi, T., Tanaka, S., Yamamoto, K., and Kikuyama, S. (2001) Gen. Comp. Endocrinol, 122, 10-16.
  32. ATP-binding cassette transporter ABC2/ABCA2 in the rat brain: A novel mammalian lysosome- associated membrane protein and a specific marker for oligodendrocytes but not for myelin sheaths.
    Zhou, C. J., Zhao, L. X., Inagaki, N., Guan, J. L., Nakajo, S., Hirabayashi, T., Kikuyama, S., and Shioda, S. (2001) J. Neurosci., 21, 849-857.
 

◆国際学会及び国外研究発表

  1. Neural immature progenitor cells have high sensitivity to methyl mercury: involvement of reactive oxidative stress.
    Watanabe, J., Ogawa, T., Naganuma, A., Shioda, S., Nakamura, M., and Nakajo, S.
    Society for Neuroscience (36th Annual Meeting)
    Oct. 14-18, 2006, Atlanta, Georgia
  2. PACAP induces differentiation of neural stem cells into astrocytes: Involvement of the β form of protein kinase C.
    Watanabe, J., Ohba, M., Ohno, F., Kikuyama, S., Nakamura, M., Shioda, S., and Nakajo, S.
    The 16th International Symposium on Regulatory Peptides (REGPEP’06), Half-day Symposium on GPCRs
    Aug. 30-Sep 2, 2006, Hakone, Japan
  3. Differentiation of neural stem cells into astrocytes by PACAP: involvement of protein kinase C in the signaling pathway.
    Watanabe, J., Kikuyama, S., Shioda, S., Nakaya, K., and Nakajo, S.
    The 7th International Symposium on VIP, PACAP and Related Peptides
    Sep. 11-15, 2005, Rouen, Normandy, France
  4. Presence and pattern of PACAP and PAC1-receptors in the central nervous system of the lumbricid oligochaete Lumbricus terrestris L.
    Molnar, L., Pollak, E., Reglodi, D., Tamas, A., Lengvari, I., Nakajo, S., Shioda, S., Arimura, A., and Lubics, A.
    The 7th International Symposium on VIP, PACAP and Related Peptides
    Sep. 11-15, 2005, Rouen, Normandy, France
  5. Pituitary adenylate cyclase-activating polypeptide (PACAP) promotes differentiation of mouse neural stem cells into astrocytes.
    Shioda, S., Watanabe, J., Kikuyama, S., Ohno, F., and Nakajo, S.
    15th International Congress of Comparative Endocrinology
    May 22-27, 2005, Boston, MA
  6. Signaling pathway of PACAP which induces induces differentiation of neural stem cells into astrocytes.
    Watanabe, J., Kikuyama, S., Shioda, S., Nakaya, K., and Nakajo, S.
    Society for Neuroscience (34th Annual Meeting)
    Oct. 23-27, 2004, San Diego, California
  7. Expression of the receptor for pituitary adenylate cyclase-activating polypeptide in the reactive astrocytes after brain injury.
    Suzuki, R., Watanabe, J., Arata, S., Nakajo, S., Kikuyama, S., and Shioda, S.
    Society for Neuroscience (34th Annual Meeting)
    Oct. 23-27, 2004, San Diego, California
  8. Expression of PACAP receptor in the reactive astrocytes after brain injury.
    Suzuki, R., Watanabe, J., Arata, S., Nakajo, S., Kikuyama, S., and Shioda, S.
    16th International Congress of the International Federation of Association of Anatomists.
    P2-180, Aug. 22-27, 2004, Kyoto, Japan
  9. Involvement of Gq signaling pathway in the differentiation of neural stem cells into astrocytes by PACAP.
    Watanabe, J., Ohno, F., Kikuyama, S., Shioda, S., Nakaya, K., and Nakajo, S.
    Showa University International Symposium for Life Sciences, 1st Annual Meeting New Frontiers in Neuroscience Research Aug. 31, 2004, Tokyo, Japan
  10. Molecular targets by an ATP-noncompetitive inhibitor of the protein tyrosin kinase, β-
    hydroxyisovalerylshikonin.
    Kajimoto, S., Horie, M., Masuda, Y., Nakajo, S., and Nakaya, K.
    2nd World Congress of the Board of Pharmaceutical Siences of FIP
    May 30-June 3, 2004, Kyoto, Japan
  11. Frizzled-3 protein expression after antidepressant treatment in rat frontal cortex.
    Iwabuchi, T., Yamada, M., Takahashi, K., Nishioka, G., Kudo, K., Kamijima, K., Yamada, M., Nakajo, S., and Momose, K.
    Society for Neuroscience (33ed Annual Meeting)
    Nov. 8-12, 2003, New Orleans, Louisiana.
  12. Low concentration of PACAP induces differentiation of mouse neural stem cells into astrocytes.
    Nakajo, S., Ohno, F., Sekihara, K., Hirabayashi, T., Shioda, S., and Nakaya, K.
    6th International Symposium on VIP, PACAP, Secretin, Glucagon and Related Peptides
    Sep. 1-4, 2003, Hakone, Japan
  13. Immunohistochemical observation of PACAP and its receptor in the rat testis.
    Funahashi, H., Kato, S., Nakajo, S., Suzuki, R., Arimura, A., and Shioda, S.
    6th International Symposium on VIP, PACAP, Secretin, Glucagon and Related Peptides
    Sep. 1-4, 2003, Hakone, Japan
  14. Up-regulation of PACAP receptor in the reactive astrocytes in mice neocortex after brain injury.
    Suzuki, R., Arata, S., Nakajo, S., Kikuyama, S., and Shioda, S.
    6th International Symposium on VIP, PACAP, Secretin, Glucagon and Related Peptides
    Sep. 1-4, 2003, Hakone, Japan
  15. PACAP prevents apoptosis of hippocampal neurons by inhibiting JNK/SAPK and p38 signal transduction pathways.
    Shioda, S., Dohi, H., Mizushima, H., Nakajo, S., Ohtaki, H., Nakamachi, T., Suzuki, R., and Funahashi, H.
    Acta Physiol. Hung, 89 (1-3), 266, 4th International Congress of Pathophysiology,
    Jun. 2002, Budapest
  16. Expression of splice variants of PAC1 receptor and its signal tranduction pathways during early neurodevelopment.
    Shioda, S., Zhou, C. J., Ohtaki, H., Funahashi, H., Yada, T., Hayashi, D., Nakajo, S., and Nakaya, K.
    5th International Symposium on VIP, PACAP, Secretin, Glucagon and Related Peptides
    Nov. 2001, Santa Barbara
  17. PACAP prevents ischemic neuronal cell death acting through IL-6.
    Ohtaki, H., Dohi, K., Mizushima, H., Takaki, A., Nakajo, S., and Shioda, S.
    5th International Symposium on VIP, PACAP, Secretin, Glucagon and Related Peptides
    Nov. 2001, Santa Barbara
  18. The role of interleukin-1after transient focal ischemia.
    Ohtaki, H., Funahashi, H., Ogawa, A., Nakajo, S., Horai, R., Asano, M., Iwakura, Y., Goto, N., Mizushima,H., and Shioda, S.
    Society for Neuroscience (31st Annual Meeting)
    Nov. 10-15, 2001, San Diego, California